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BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) <t>fluorescent</t> probe, accompanied by the relevant quantitative determination. ( b , d ) <t>Superoxide</t> anion (O 2 − ) production in irradiated BMSCs was monitored with the <t>DHE</t> fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.
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BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) <t>fluorescent</t> probe, accompanied by the relevant quantitative determination. ( b , d ) <t>Superoxide</t> anion (O 2 − ) production in irradiated BMSCs was monitored with the <t>DHE</t> fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.
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dihydroethidium dhe fluorescent probe  (Applygen Technologies)
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BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) <t>fluorescent</t> probe, accompanied by the relevant quantitative determination. ( b , d ) <t>Superoxide</t> anion (O 2 − ) production in irradiated BMSCs was monitored with the <t>DHE</t> fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.
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BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) fluorescent probe, accompanied by the relevant quantitative determination. ( b , d ) Superoxide anion (O 2 − ) production in irradiated BMSCs was monitored with the DHE fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.

Journal: Bioengineering

Article Title: Fe 3 O 4 Magnetic Nanoparticles and Static Magnetic Field Stimulated BMSC-Derived Exosomes Promoted Osteogenesis and Alleviated Oxidative Stress in Irradiated BMSCs Through miR-429/NOG Pathway

doi: 10.3390/bioengineering13040402

Figure Lengend Snippet: BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) fluorescent probe, accompanied by the relevant quantitative determination. ( b , d ) Superoxide anion (O 2 − ) production in irradiated BMSCs was monitored with the DHE fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.

Article Snippet: Similarly, intracellular superoxide anion levels were measured using a Dihydroethidium (DHE) Superoxide Anion Fluorescent Probe Kit (G1904-100T, Servicebio, Wuhan, Hubei, China) in full accordance with the manufacturer’s instructions.

Techniques: Irradiation, Concentration Assay